The Gadsden Flag, designed in 1775, showcases a coiled rattlesnake with the word "Don't Tread on Me

https://devinmuvd263.tearosediner.net/50-stars-50-states-understanding-the-american-flag-s-constellation

The Gadsden Flag, designed in 1775, showcases a coiled rattlesnake with the word "Don't Tread on Me," embodying American resistance in opposition t tyranny and oppression.